Professor Brian Forde

Professor

Research Interests

The molecular genetics of plant-nutrient interactions. Of the many functions performed by plant roots, the most important is the extraction of water and nutrients from the soil. To achieve this efficiently in the face of competition from other organisms and physical processes such as leaching, roots have acquired a sophisticated suite of adaptations. Understanding the molecular basis of these adaptations will be important for improving crucial agronomic traits such as nutrient-use efficiency. We are particularly interested in the molecular processes that underlie patterns of ?foraging? behaviour in plant roots. The efficiency with which roots explore the soil is greatly enhanced by their ability to preferentially colonise nutrient-rich soil patches. Using Arabidopsis as a model, we previously identified a MADS-box gene (ANR1) which is a key component of a signalling pathway that enables root growth to respond positively to the external presence of nitrate. By engineering transgenic lines in which ANR1 over-expression is dexamethasone-inducible, we have shown that up-regulation of ANR1 in one part of the root system is sufficient to initiate a ?foraging? response, even in the absence of nitrate (unpublished results). Recent progress, in collaboration with Alain Gojon?s group at INRA-Montpellier, suggests that the identity of the long sought-after ?nitrate sensor? that lies upstream of ANR1 may now be known. Current evidence suggests it takes the form of a protein previously known for its role as a dual-affinity nitrate transporter, NRT1.1. Other work is looking at the ability of root tips to respond to external signals from the amino acid L-glutamate (L-Glu). It now appears that the external presence of L-Glu (but not other amino acids) is able to induce major changes in Arabidopsis root architecture that are quite distinct from those elicited by nitrate. Using a combination of approaches, including QTL mapping and forward and reverse genetics, we are trying to determine the molecular basis of the root?s ability to sense external L-Glu. A long-term goal is to use the knowledge gained in these studies to develop plant varieties that are more efficient at using fertilisers, so reducing costs and minimising the environmental impact of arable farming.

Glutamate receptor-like channels in plants: an amino acid sensing role in plant defence?
Forde, B., Roberts, M. 2/06/2014
Scientific review

Glutamate signalling in roots
Forde, B. 2014 In: Journal of Experimental Botany. 65, 3, p. 779-787. 9 p.
Journal article

Glutamate signalling via a MEKK1 kinase-dependent pathway induces changes in Arabidopsis root architecture
Forde, B., Cutler, S., Zaman, N., Krysan, P. 07/2013 In: The Plant journal : for cell and molecular biology. 75, 1, p. 1-10. 10 p.
Journal article

Overexpressing the ANR1 MADS-Box Gene in Transgenic Plants Provides New Insights into its Role in the Nitrate Regulation of Root Development
Gan, Y., Bernreiter, A., Filleur, S., Abram, B., Forde, B.G. 06/2012 In: Plant and Cell Physiology. 53, 6, p. 1003-1016. 14 p.
Journal article

Quantitative analysis of lateral root development: pitfalls and how to avoid them
Dubrovsky, J.G., Forde, B.G. 01/2012 In: Plant Cell. 24, 1, p. 4-14. 11 p.
Journal article

Nitrate and glutamate as environmental cues for behavioural responses in plant roots
Forde, B., Walch-Liu, P. 06/2009
Scientific review

Is it good noise?: The role of developmental instability in the shaping of a root system
Forde, B.G. 2009 In: Journal of Experimental Botany. 60, 14, p. 3989-4002. 14 p.
Journal article

Nitrate signalling mediated by the NRT1.1 nitrate transporter antagonises L-glutamate-induced changes in root architecture
Walch-Liu, P., Forde, B.G. 06/2008 In: The Plant journal : for cell and molecular biology. 54, 5, p. 820-828. 9 p.
Journal article

Gene expression, cellular localisation and function of glutamine synthetase isozymes in wheat (Triticum aestivum L.)
Bernard, S.M., Møller, A.L.B., Dionisio, G., Kichey, T., Jahn, T.P., Dubois, F., Baudo, M., Lopes, M.S., Tercé-Laforgue, T., Foyer, C.H., Parry, M.A.J., Forde, B.G., Araus, J.L., Hirel, B., Schjoerring, J.K., Habash, D.Z. 2008 In: Plant Molecular Biology. 67, 1-2, p. 89-105. 18 p.
Journal article

Glutamate in plants: metabolism, regulation and signalling
Forde, B., Lea, P. 2007
Scientific review

L-Glutamate as a novel modifier of root growth and branching: What's the sensor?
Walch-Liu, P., Forde, B. 2007
Scientific review

The Arabidopsis NRT1.1 transporter participates in the signalling pathway triggering root colonisation of nitrate-rich patches.
Remans, T., Nacry, P., Pervent, M., Filleur, S., Diatloff, E., Mounier, E., Tillard, P., Forde, B.G., Gojon, A. 12/12/2006 In: Proceedings of the National Academy of Sciences. 103, 50, p. 19206-19211. 6 p.
Journal article

Expression and transport characterisation of the wheat low-affinity cation transporter (LCT1) in the methylotrophic yeast Pichia pastoris.
Diatloff, E., Forde, B.G., Roberts, S.K. 9/06/2006 In: Biochemical and Biophysical Research Communications. 344, 3, p. 807-813. 7 p.
Journal article

Nitrogen regulation of root branching.
Walch-Liu, P., Ivanov, I.I., Filleur, S., Gan, Y., Remans, T., Forde, B.G. 05/2006 In: Annals of Botany. 97, 5, p. 875-881. 7 p.
Journal article

Nitrate assimilation in the forage legume Lotus japonicus L.
Prosser, I.M., Massonneau, A., Smyth, A.J., Waterhouse, R.N., Forde, B.G., Clarkson, D.T. 03/2006 In: Planta. 223, 4, p. 821-834. 14 p.
Journal article

Evidence that L-glutamate can act as an exogenous signal to modulate root growth and branching in Arabidopsis thaliana.
Walch-Liu, P., Liu, L., Remans, T., Tester, M., Forde, B.G. 2006 In: Plant and Cell Physiology. 47, 8, p. 1045-1057. 13 p.
Journal article

Plant methods moves to fund open-access publishing
Forde, B.G., Roberts, M.R. 2006 In: Plant Methods. 2, 6
Editorial

Nutritional regulation of ANRI and other root-expressed MADS-box genes in Arabidopsis thaliana.
Gan, Y.B., Filleur, S., Rahman, A., Gotensparre, S., Forde, B.G. 11/2005 In: Planta. 222, 4, p. 730-742. 13 p.
Journal article

What is apical and what is basal in plant root development?
Baluška, F., Barlow, P.W., Baskin, T.I., Chen, R.J., Feldman, L., Forde, B.G., Geisler, M., Jernstedt, J., Menzel, D., Muday, G.K., Murphy, A., Šamaj, J., Volkmann, D. 09/2005 In: Trends in Plant Science. 10, 9, p. 409-411. 3 p.
Journal article

Plant methods: putting the spotlight on technological innovation in the plant sciences.
Forde, B.G., Roberts, M.R. 18/08/2005 In: Plant Methods. 1, 1, 1 p.
Editorial

Nitrate and glutamate sensing by root plants.
Filleur, S., Walch-Liu, P., Gan, Y.B., Forde, B.G. 02/2005 In: Biochemical Society Transactions. 33, 1, p. 283-286. 4 p.
Journal article

Signalling mechanisms integrating root and shoot responses to changes in the nitrogen supply.
Walch-Liu, P., Filleur, S., Gan, Y.B., Forde, B.G. 02/2005 In: Photosynthesis Research. 83, 2, p. 239-250. 12 p.
Journal article

Transcriptome analysis of root transporters reveals participation of multiple gene families in the response to cation stress.
Maathuis, F.J.M., Filatov, V., Herzyk, P., Krijger, G.C., Axelsen, K.B., Chien, S.X., Green, B.J., Li, Y., Madagan, K.L., Sanchez-Fernandez, R., Forde, B.G., Palmgren, M.G., Rea, P.A., Williams, L.E., Sanders, D., Amtmann, A. 09/2003 In: Plant Journal. 35, 6, p. 675-692. 18 p.
Journal article

Nitrate finds a place in the sun.
Forde, B.G., Cole, J.A. 02/2003 In: Plant Physiology. 131, 2, p. 395-400. 6 p.
Journal article

Local and long-range signalling pathways regulating plant responses to nitrate.
Forde, B.G. 06/2002 In: Annual Review of Plant Biology. 53, p. 203-224. 22 p.
Journal article

The role of long-distance signalling in plant responses to nitrate and other nutrients.
Forde, B.G. 01/2002 In: Journal of Experimental Botany. 53, 366, p. 39-43. 5 p.
Journal article

Molecular and developmental biology of inorganic nirogen nutrition.
Crawford, N.M., Forde, B.G. 2002 In: The Arabidopsis Book. Rockville, Md. : American Society of Plant Biologists
Chapter

Nitrate uptake and its regulation.
Touraine, B., Daniel-Vedele, F., Forde, B.G. 2001 In: Plant Nitrogen. Berlin : Springer Verlag p. 1-36. 36 p.
Chapter

The nutritional control of root development.
Forde, B.G., Lorenzo, H. 2001 In: Plant Soil. 232, p. 51-68. 18 p.
Journal article

A fungal nitrate carrier with two transport mechanisms.
Zhou, J., Trueman, L.J., Boorer, K.J., Theodoulou, F.L., Forde, B.G., Miller, A.J. 22/12/2000 In: Journal of Biological Chemistry. 275, 51, p. 39894-39899. 6 p.
Journal article

Hormonal regulation of axillary bud growth in Arabidopsis.
Chatfield, S.P., Stirnber, G.P., Forde, B.G., Leyser, H.M.O. 10/2000 In: Plant Journal. 24, 2, p. 159-169. 11 p.
Journal article

Nitrate transporters in plants : structure, function and regulation.
Forde, B.G. 1/05/2000 In: Biochimica et Biophysica Acta (BBA) - Biomembranes. 1465, 1-2, p. 219-235. 17 p.
Journal article

Regulation of Arabidopsis root development by nitrate availability.
Zhang, H., Forde, B.G. 01/2000 In: Journal of Experimental Botany. 51, 342, p. 51-59. 9 p.
Journal article

Dual pathways for regulation of root branching by nitrate.
Zhang, H., Jennings, A., Barlow, P., Forde, B.G. 25/03/1999 In: Proceedings of the National Academy of Sciences. 96, 11, p. 6529-6534. 6 p.
Journal article

Nucleotide sequence of a cDNA encoding an Arabidopsis urease accessory protein (accession number AF109374).
Freyermuth, S.K., Forde, B.G., Polacco, J.C. 01/1999 In: Plant Physiology. 119, 1, p. 364.
Journal article

Nitrate and ammonium nutrition of plants : physiological and molecular perspectives.
Forde, B.G., Clarkson, D.T. 1999 In: Advances in Botanical Research. 30, p. 1-90. 90 p.
Journal article

The use of mutants and transgenic plants to study amino acid metabolism.
Lea, P.J., Forde, B.G. 1994 In: Plant, Cell and Environment. 17, 5, p. 541-556. 16 p.
Journal article