Dr Stephen Roberts

Lecturer

Research Overview

My research expertise is the application of electrophysiological and molecular biological techniques to understand the regulation of ion transport across the biological membranes of plant and fungal cells. I currently use the filamentous fungus, Aspergillus nidulans and the yeast, Saccharomyces cerevisiae. My research interests have more recently focussed on anion efflux and Ca2+ influx across the plasma membrane of fungal cells towards identifying and evaluating potential new drug targets.

More recently I have collaborated with collegues in the Physics Department (Lancaster University) to investigate the role of transmembrane voltage in signal transduction and mechanisms underpinning selectivity of ion channels

Ionic Coulomb blockade and anomalous mole fraction effect in the NaChBac bacterial ion channel and its charge-varied mutants
Kaufman, I.K., Fedorenko, O., Luchinsky, D., Gibby, W., Roberts, S.K., McClintock, P.V.E., Eisenberg, R.S. 11/09/2017 In: EPJ Nonlinear Biomedical Physics. 5, 8 p.
Journal article

Sodium binding sites and permeation mechanism in the NaChBac channel: a molecular dynamics study
Guardiani, C., Rodger, P.M., Fedorenko, O., Roberts, S.K., Khovanov, I.A. 14/03/2017 In: Journal of Chemical Theory and Computation. 13, 3, p. 1389-1400. 12 p.
Journal article

Ionic Coulomb blockade and anomalous mole fraction effect in NaChBac bacterial ion channels
Kaufman, I.K., Fedorenko, O.A., Luchinsky, D.G., Gibby, W.A.T., Roberts, S., McClintock, P.V.E., Eisenberg, R.S. 8/12/2016 In: arXiv.
Journal article

Valproate inhibits MAP kinase signalling and cell cycle progression in S. cerevisiae.
Desfossés-Baron, K., Hammond-Martel, I., Simoneau, A., Sellam, A., Roberts, S.K., Wurtele, H. 26/10/2016 In: Scientific Reports. 6, p. 1-14. 14 p.
Journal article

Calcium dependence of Eugenol tolerance and toxicity in Saccharomyces cerevisiae
Roberts, S., McAinsh, M., Cantopher, H., Sandison, S. 18/07/2014 In: PLoS ONE. 9, 7, 8 p.
Journal article

Calcium imaging of the cyclic nucleotide response
McAinsh, M., Roberts, S., Dubovskaya, L. 2013 In: Cyclic nucleotide signalling in plants. New York : Humana Press p. 107-119. 13 p. ISBN: 9781627034401. Electronic ISBN: 9781627034418.
Chapter

Cch1p mediates Ca2+ influx to protect Saccharomyces cerevisiae against eugenol toxicity
Roberts, S., McAinsh, M., Widdicks, L. 13/09/2012 In: PLoS ONE. 7, 9, 7 p.
Journal article

Characterisation of AnBEST1, a functional anion channel in the plasma membrane of the filamentous fungus, Aspergillus nidulans.
Roberts, S., Milnes, J., Caddick, M. 09/2011 In: Fungal Genetics and Biology. 48, 9, p. 928-938. 10 p.
Journal article

A CLC chloride channel plays an essential role in copper homeostasis in Aspergillus nidulans at increased extracellular copper concentrations.
Odden, D.M., Diatloff, E., Roberts, S.K. 1/10/2007 In: Biochimica et Biophysica Acta (BBA) - Biomembranes. 1768, 10, p. 2466-2477. 12 p.
Journal article

Expression and transport characterisation of the wheat low-affinity cation transporter (LCT1) in the methylotrophic yeast Pichia pastoris.
Diatloff, E., Forde, B.G., Roberts, S.K. 9/06/2006 In: Biochemical and Biophysical Research Communications. 344, 3, p. 807-813. 7 p.
Journal article

Plasma membrane anion channels in higher plants and their putative functions in roots.
Roberts, S.K. 03/2006 In: New Phytologist. 169, 4, p. 647-666. 20 p.
Journal article

The Saccharomyces cerevisiae Ca2+ channel Cch1pMid1p is essential for tolerance to cold stress and iron toxicity.
Peiter, E., Fischer, M., Sidaway, K., Roberts, S.K., Sanders, D. 24/10/2005 In: FEBS Letters. 579, 25, p. 5697-5703. 7 p.
Journal article

Phosphate-dependent anion channel-mediated citrate efflux from Arabidopsis roots
Diatloff, E., Roberts, M., Sanders, D., Roberts, S. 07/2005
Meeting abstract

The Saccharomyces cerevisiae Ca2+ channel Cch1pMid1p reveals a role for Ca2+ influx in iron tolerance
Peiter, E., Fischer, M., Sidaway, K., Roberts, S.K., Sanders, D. 07/2005
Meeting abstract

Characterization of Anion Channels in the Plasma Membrane of Arabidopsis Epidermal Root Cells and the Identification of a Citrate-Permeable Channel Induced by Phosphate Starvation.
Diatloff, E., Roberts, M., Sanders, D., Roberts, S.K. 12/2004 In: Plant Physiology. 136, 4, p. 4136-4149. 14 p.
Journal article

Differential regulation of K+ channels in Arabidopsis epidermal and stelar root cells.
Diatloff, E., Geiger, D., Shang, L., Hedrich, R., Roberts, S.K. 08/2004 In: Plant, Cell and Environment. 27, 8, p. 980-990. 11 p.
Journal article

Anion channels in roots : a role in sulphate transport.
Diatloff, E., Brown, R., Roberts, S.K. 03/2004 In: Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology. 137, 3 Supp, p. S240.
Journal article

Investigation into the functions of CLC channels in the filamentous fungus Aspergillus nidulans.
Oddon, E., Diatloff, E., Roberts, S.K. 03/2004 In: Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology. 137, 3 Supp, p. S196-S197.
Journal article

Mitochondria provide the main source of cytosolic ATP for activation of outward-rectifying K+ channels in mesophyll protoplast of chlorophyll-deficient mutant rice (OsCHLH) seedlings.
Goh, C., Jung, K., Roberts, S.K., McAinsh, M., Hetherington, A.M., Park, Y.I., Suh, K.H. 20/02/2004 In: Journal of Biological Chemistry. 279, 8, p. 6874-6882. 9 p.
Journal article

TOK homologue in Neurospora crassa : first cloning and functional characterisation of an ion channel in a filamentous fungus.
Roberts, S.K. 02/2003 In: Eukaryotic Cell. 2, 1, p. 181-190. 10 p.
Journal article

A Novel Low-Affinity H⁺-Cl⁻ Co-Transporter in Yeast: Characterization by Patch Clamp
Roberts, S.K., Dixon, G.K., Fischer, M., Sanders, D. 07/2001 In: Mycologia. 93, 4, p. 636-633. 4 p.
Journal article

The effects of ABA on channel mediated K+ transport across higher plant roots.
Roberts, S.K., Snowman, B.N. 09/2000 In: Journal of Experimental Botany. 51, 350, p. 1585-1594. 10 p.
Journal article

Divalent Cation Block of Inward Currents and Low-Affinity K+ Uptake in Saccharomyces cerevisiae.
Roberts, S.K., Fischer, M., Dixon, G.K., Sanders, D. 01/1999 In: Journal of Bacteriology. 181, 1, p. 291-297. 7 p.
Journal article

Divalent cation block of inward currents and low-afinity K+ uptake in Saccharomyces cerevisiae
Roberts, S.K., Fischer, M., Dixon, G.K., Sanders, D. 01/1999 In: Journal of Bacteriology. 181, 1, p. 291-297. 7 p.
Journal article

Regulation of K+ channels in maize roots by water stress and abscisic acid
Roberts, S.K. 01/1998 In: Plant Physiology. 116, 1, p. 145-153. 9 p.
Journal article

Integration of ion channel activity in calcium signalling pathways
Sanders, D., Allen, G.J., Muir, S.R., Roberts, S.K. 1998 In: Cellular Integration of Signalling Pathways in Plant Development. Berlin : Springer Verlag p. 47-58. 12 p.
Paper

Laser ablation of the cell wall and localized patch clamping of the plasma membrane in the filamentous fungus Aspergillus: characterization of an anion-selective efflux channel
Roberts, S.K., Dixon, G.K., Dunbar, S.J., Sanders, D. 12/1997 In: New Phytologist. 137, 4, p. 579-585. 7 p.
Journal article

Permeation of Ca2+ and monovalent cations through an outwardly rectifying channel in maize root stelar cells
Roberts, S.K., Tester, M. 04/1997 In: Journal of Experimental Botany. 48, 309, p. 839-846. 8 p.
Journal article

A patch clamp study of Na+ transport in maize roots
Roberts, S.K., Tester, M. 03/1997 In: Journal of Experimental Botany. 48, p. 431-440. 10 p.
Journal article

Inward and outward K+-selective currents in the plasma membrane of protoplasts from maize root cortex and stele
Roberts, S.K., Tester, M. 12/1995 In: The Plant Journal. 8, 6, p. 811-825. 15 p.
Journal article

Cytoplasmic calcium and Fucus egg activation
Roberts, S., Gillot, I., Brownlee, C. 01/1994 In: Development. 120, 1, p. 155-163. 9 p.
Journal article

The role of Ca2+ in signal-transduction following fertilization in Fucus serratus
Roberts, S., Berger, F., Brownlee, C. 1/11/1993 In: Journal of Experimental Biology. 184, p. 197-212. 16 p.
Journal article

Calcium and related channels in fertilization and early development of Fucus
Taylor, A.R., Roberts, S., Brownlee, C. 29/10/1992 In: Philosophical Transactions B: Biological Sciences. 338, 1283, p. 97-104. 8 p.
Journal article