Dr Paul McKean

Senior Lecturer

Research Overview

My research expertise is molecular and cellular parasitology. The main focus of my research at present is the African trypanosome, Trypanosoma brucei; the causative agent of human sleeping sickness and the cattle wasting disease Nagana in sub-Saharan Africa. I have specific interests in (i) understanding the role of novel microtubule associated proteins in regulating cytoskeletal dynamics and cellular morphogenesis and (ii) identifying how proteins are specifically targeted to, and subsequently assembled into, the trypanosome flagellum.

Variation in Basal Body Localisation and Targeting of Trypanosome RP2 and FOR20 Proteins
Harmer, J., Qi, X., Toniolo, G., Patel, A., Shaw, H., Benson, F.E., Ginger, M.L., McKean, P.G. 08/2017 In: Protist. 168, 4, p. 452-466. 15 p.
Journal article

Modulation of flagellum attachment zone protein FLAM3 and regulation of the cell shape in Trypanosoma brucei life cycle transitions
Sunter, J.D., Benz, C., Andre, J., Whipple, S., McKean, P.G., Gull, K., Ginger, M.L., Lukeš, J. 15/08/2015 In: Journal of Cell Science. 128, 16, p. 3117-3130. 14 p.
Journal article

Eukaryotic flagella: variations in form, function, and composition during evolution
Moran, J., McKean, P., Ginger, M. 1/12/2014 In: Bioscience. 64, 12, p. 1103-1114. 12 p.
Journal article

An alternative model for the role of RP2 in flagellum assembly in the African trypanosome: RP2 function in T. brucei
Andre, J., Kerry, L., Qi, X., Hawkins, E., Drižytė, K., Ginger, M., McKean, P. 3/01/2014 In: Journal of Biological Chemistry. 289, 1, p. 464 –475. 12 p.
Journal article

Tubulin-binding cofactor C domain-containing protein TBCCD1 orchestrates cytoskeletal filament formation
Andre, J., Harrison, S., Towers, K., Qi, X., Vaughan, S., McKean, P., Ginger, M. 12/2013 In: Journal of Cell Science. 126, p. 5350-5356. 7 p.
Journal article

Proteomic insights into parasite biology
Ginger, M.L., McKean, P.G., Burchmore, R., Grant, K.M. 08/2012 In: Parasitology. 139, 9, p. 1101-1102. 2 p.
Journal article

Flagellum function and assembly during the Leishmania life cycle.
Gluenz, E., Ginger, M.L., McKean, P.G. 08/2010 In: Current Opinion in Microbiology. 13, 4, p. 473-479. 7 p.
Journal article

The flagellar pocket of trypanosomatids: a critical feature for cell morphogenesis and pathogenicity
McKean, P., Gull, K. 2010 In: Structures and organelles in pathogenic protists. Berlin : Springer Verlag p. 87-113. 27 p.
Chapter (peer-reviewed)

Analysis of the trypanosome flagellar proteome using a combined electron transfer collisionally activated dissociation strategy.
Hart, S.R., Wai Lau, K.W., Hao, Z., Broadhead, R., Portman, N., Huhmer, A., Gull, K., McKean, P.G., Hubbard, S.J., Gaskell, S.J. 02/2009 In: Journal of The American Society for Mass Spectrometry. 20, 2, p. 167-175. 9 p.
Journal article

Combining RNA interference mutants and comparative proteomics to identify protein component interactions and dependencies in a eukaryotic flagellum.
Portman, N., Lacomble, S., Thomas, B., McKean, P.G., Gull, K. 02/2009 In: Journal of Biological Chemistry. 284, 9, p. 5610-5619. 10 p.
Journal article

Swimming with protists : perception, motility and flagellum assembly.
Ginger, M.L., Portman, N., McKean, P.G. 11/2008 In: Nature Reviews in Microbiology. 6, 11, p. 838-850. 13 p.
Journal article

An essential quality control mechanism at the eukaryotic basal body prior to intraflagellar transport.
Stephan, A., Vaughan, S., Shaw, M.K., Gull, K., McKean, P.G. 10/2007 In: Traffic. 8, 10, p. 1323-1330. 8 p.
Journal article

Cell structure, cell division and cell cycle.
Hammarton, T., Wickstead, B., McKean, P.G. 2007 In: Trypanosomes : after the genome. Wymondham : Horizon Bioscience p. 239-280. 42 p. ISBN: 978-1-904933-27-4.
Chapter

Basal body and flagellum mutants reveal a rotational constraint of the central pair microtubules in the axonemes of trypanosomes.
Gadelha, C., Wickstead, B., McKean, P.G., Gull, K. 15/06/2006 In: Journal of Cell Science. 119, 12, p. 2405-2413. 9 p.
Journal article

Flagellar motility is required for the viability of the bloodstream trypanosome.
Broadhead, R., Dawe, H.R., Farr, H., Griffiths, S., Hart, S.R., Portman, N., Shaw, M.K., Ginger, M.L., Gaskell, S.J., McKean, P.G., Gull, K. 9/03/2006 In: Nature. 440, 7081, p. 224-227. 4 p.
Journal article

Trypanosome IFT mutants provide insight into the motor location for mobility of the flagella connector and flagella membrane formation.
Davidge, J.A., Shawcross, E., Dickinson, H.A., Towers, K., Ginger, M.L., McKean, P.G., Gull, K. 2006 In: Journal of Cell Science. 119, 19, p. 3935-3943. 9 p.
Journal article

The flagella connector of Trypanosoma brucei : an unusual mobile transmembrane junction.
Briggs, L., McKean, P.G., Baines, A., Moreira-Leite, F., Davidge, J., Vaughan, S., Gull, K. 04/2004 In: Journal of Cell Science. 117, 9, p. 1641-1651. 11 p.
Journal article

Coordination of cell cycle progression and cytokinesis in Trypanosoma brucei.
McKean, P.G. 12/2003 In: Current Opinion in Microbiology. 6, 6, p. 600-607. 8 p.
Journal article

γ-Tubulin Functions in the Nucleation of a Discrete Subset of Microtubules in the Eukaryotic Flagellum.
McKean, P.G., Baines, A., Vaughan, S., Gull, K. 1/04/2003 In: Current Biology. 13, 7, p. 598-602. 5 p.
Journal article

Myristoyl-CoA:Protein N-Myristoyltransferase, an Essential Enzyme and Potential Drug Target in Kinetoplastid Parasites.
Price, H., Menon, M.R., Panethymitaki, C., Goulding, D., McKean, P.G., Smith, D.F. 28/02/2003 In: Journal of Biological Chemistry. 278, 9, p. 7206-7214. 9 p.
Journal article

Plasmodium chabaudi chabaudi AS: modification of acute infection in CBA/Ca mice as a result of pre-treatment with erythrocyte band 3 in adjuvant.
O'Dea, K.P., McKean, P.G., Brown, K.N. 10/2002 In: Experimental Parasitology. 102, 2, p. 66-71. 6 p.
Journal article

Phenotypic changes associated with deletion and over-expression of a stage-regulated gene family in Leishmania.
McKean, P.G., Denny, P.W., Kneupfer, E., Keen, J.K., Smith, D.F. 08/2001 In: Cellular Microbiology. 3, 8, p. 511-523. 13 p.
Journal article

The extended tubulin superfamily.
McKean, P.G., Vaughan, S., Gull, K. 08/2001 In: Journal of Cell Science. 114, 15, p. 2723-2733. 11 p.
Journal article

Identification and characterisation of a RAD51 gene from Leishmania major.
McKean, P.G., Keen, J.G., Smith, D.F., Benson, F.E. 07/2001 In: Molecular and Biochemical Parasitology. 115, 2, p. 209-216. 8 p.
Journal article

Characterisation of a differentially expressed protein that shows an unusual localisation to intracellular membranes in Leishmania major.
Kneupfer, E., Stierhof, Y.D., McKean, P.G., Smith, D.F. 1/06/2001 In: Biochemical Journal. 356, 2, p. 335-344. 10 p.
Journal article

Molecular parasitology : new insights.
McKean, P.G., Keen, J.K., Kelly, J.M., Smith, D.F. 12/2000 In: Parasitology Today. 15, 12, p. 469-470. 2 p.
Journal article

New tubulins in protozoal parasites.
Vaughan, S., Attwood, T., Navarro, M., Scott, V., McKean, P.G., Gull, K. 2000 In: Current Biology. 10, 7, p. R258-259.
Journal article

Diversity in repeat-containing surface proteins of Leishmania major.
McKean, P.G., Trenholme, K.R., Rangarajan, D., Keen, J.K., Smith, D.F. 06/1997 In: Molecular and Biochemical Parasitology. 86, 2, p. 225-235. 11 p.
Journal article

Characterisation of a second protein encoded by the differentially regulated LmcDNA16 gene family of Leishmania major.
McKean, P.G., Delahay, R., Pimenta, P.F., Smith, D.F. 04/1997 In: Molecular and Biochemical Parasitology. 85, 2, p. 221-231. 11 p.
Journal article

A single gene copy merozoite surface antigen and immune evasion?
O’Dea, K.P., McKean, P.G., Jarra, W., Brown, K.N. 04/1996 In: Parasite Immunology. 18, 4, p. 165-172. 8 p.
Journal article

Processing of the Plasmodium chabaudi chabaudi AS merozite surface protein 1 in vivo and in vitro.
O’Dea, K.P., McKean, P.G., Harris, A., Brown, K.N. 06/1995 In: Molecular and Biochemical Parasitology. 72, 1-2, p. 111-119. 9 p.
Journal article

Structure and function of infective stage proteins of Leishmania.
Smith, D.F., Gokool, S., Keen, J.K., McKean, P.G., Rangarajan, D. 1994 In: Biochemical Society Transactions. 22, 2, p. 286-291. 6 p.
Journal article

A single amino acid determines the specificity of a monoclonal antibody which inhibits Plasmodium chabaudi AS in vivo.
McKean, P.G., O'Dea, K.P., Brown, K.N. 12/1993 In: Molecular and Biochemical Parasitology. 62, 2, p. 211-221. 11 p.
Journal article

Nucleotide sequence analysis and epitope mapping of the merozoite surface protein 1 from Plasmodium chabaudi chabaudi AS.
McKean, P.G., O’Dea, K.P., Brown, K.N. 12/1993 In: Molecular and Biochemical Parasitology. 62, 2, p. 199-209. 11 p.
Journal article

Antigenic cross-reactivity between Necator americanus and Ascaris lumbricoides in a community in Papua New Guinea infected predominantly with hookworm.
Pritchard, D.I., Quinnell, R.J., McKean, P.G., Walsh, L., Leggett, K.V., Slater, A.F., Raiko, A., Dale, D.D., Keymer, A.E. 07/1991 In: Transactions of The Royal Society of Tropical Medicine and Hygiene. 85, 4, p. 511-514. 4 p.
Journal article

Necator americanus secretory acetylcholinesterase and its purification from excretory-secretory products by affinity chromatography.
Pritchard, D.I., Leggett, K.V., Rogan, M.T., McKean, P.G., Brown, A. 03/1991 In: Parasite Immunology. 13, 2, p. 187-199. 13 p.
Journal article

Hookworm (Necator americanus) infection and storage iron depletion.
Pritchard, D.I., Quinnell, R.J., Moustafa, M., McKean, P.G., Slater, A.F., Raiko, A., Dale, D.D., Keymer, A.E. 1991 In: Transactions of The Royal Society of Tropical Medicine and Hygiene. 85, 2, p. 235-238. 4 p.
Journal article

Recent and predicted advances in hookworm biology.
Pritchard, D.I., McKean, P.G., Quinnell, R.J., Tighe, P.J. 1991 In: Parasitic Nematodes: Membranes, Antigens and Genes.. London : Taylor & Francis p. 140-169. 30 p. ISBN: 0850667720.
Chapter

The epidemiological significance of the immune response to the cuticular collagen of Necator americanus : a preliminary study in a hookworm-endemic area in Papua New Guinea.
Pritchard, D.I., Quinnell, R.J., Slater, A.F., McKean, P.G., Dale, D.D., Raiko, A., Keymer, A.E. 07/1990 In: Acta Tropica. 47, 5-6, p. 403-407. 5 p.
Journal article

An immunological and biochemical comparison of hookworm species.
Pritchard, D.I., McKean, P.G., Schad, G.A. 05/1990 In: Parasitology Today. 6, 5, p. 154-156. 3 p.
Journal article

The identification of a species-specific antigen from Necator americanus.
Pritchard, D.I., McKean, P.G., Rogan, M.T., Schad, G.A. 05/1990 In: Parasite Immunology. 12, 3, p. 259-267. 9 p.
Journal article

Epidemiology and immunology of Necator americanus infection in a community in Papua New Guinea: humoral responses to excretory-secretory and cuticular collagen antigens.
Pritchard, D.I., Quinnell, R.J., Slater, A.F., McKean, P.G., Dale, D.D., Raiko, A., Keymer, A.E. 1990 In: Parasitology. 100, 2, p. 317-326. 10 p.
Journal article

The action of a mast cell protease on the cuticular collagens of Necator americanus.
McKean, P.G., Pritchard, D.I. 05/1989 In: Parasite Immunology. 11, 3, p. 293-297. 5 p.
Journal article

Cuticular collagens – a concealed target for immune attack in hookworms.
Pritchard, D.I., McKean, P.G., Rogan, M.T. 09/1988 In: Parasitology Today. 4, 9, p. 239-241. 3 p.
Journal article

Cuticle preparations from Necator americanus and their immunogenicity in the infected host.
Pritchard, D.I., McKean, P.G., Rogan, M.T. 04/1988 In: Molecular and Biochemical Parasitology. 28, 3, p. 275-283. 9 p.
Journal article