Dr Douglas OrrSenior Research Associate
My work as a member of the Photosynthesis Lab at LEC centres on the central enzyme of photosynthesis, Rubisco (Ribulose 1,5-bisphosphate carboxylase/oxygenase). As the gatekeeper of carbon entry into the biosphere, Rubisco serves the critical role of assimilating carbon from atmospheric CO2 for the plant to use in growth. However, Rubisco has undesirable characteristics which make it a major rate-limiter of photosynthesis, particularly in crop plants. As part of RIPE: Realizing Increased Photosynthetic Efficiency, we are investigating Rubisco diversity and it's regulation during rapid changes in conditions. We are also collaborating with colleagues to investigate the incorporation of cyanobacterial carbon concentrating mechanisms into higher plants as a means to overcome some of Rubiscos shortcomings and boost plant productivity.
My current research contributes to projects studying the implementation of a cyanobacterial CCM into higher plants, Rubisco catalytic diversity, and Rubisco regulation under fluctuating conditions.
I am working with Martin Parry and Elizabete Carmo-Silva, as well as other members of the photosynthesis team here at LEC. I also have and am part of active collaborations with colleagues at the University of Illinois (US), Cornell, Glasgow University, USDA-ARS, ITQB-UNL (Portugal), ANU (Aus) and others.
Extraction of Rubisco to Determine Catalytic Constants
Orr, D.J., Carmo-Silva, A.E. 22/05/2018 In: Photosynthesis. Springer
Engineering photosynthesis: progress and perspectives
Orr, D.J., Pereira, A., da Fonseca Pereira, P., Pereira-Lima, I., Zsogon, A., Araujo, W. 26/10/2017
Uncertainty in measurements of the photorespiratory CO2 compensation point and its impact on models of leaf photosynthesis
Walker, B., Orr, D.J., Carmo-Silva, A.E., Parry, M.A.J., Bernacchi, C.J., Ort, D.R. 06/2017 In: Photosynthesis Research. 132, 3, p. 245-255. 11 p.
Rubisco small subunits from the unicellular green alga Chlamydomonas complement Rubisco-deficient mutants of Arabidopsis
Atkinson, N., Leitão, N., Orr, D.J., Meyer, M., Carmo-Silva, A.E., Griffiths, H., Smith, A., McCormick, A. 04/2017 In: New Phytologist. 214, 2, p. 655-667. 13 p.
Surveying Rubisco diversity and temperature response to improve crop photosynthetic efficiency
Orr, D.J., Melao Alcantara, A., Kapralov, M.V., Andralojc, P.J., Carmo-Silva, A.E., Parry, M.A.J. 3/10/2016 In: Plant Physiology. 172, 2, p. 707-717. 11 p.
Rubisco catalytic properties of wild and domesticated relatives provide scope for improving wheat photosynthesis
Prins, A., Orr, D.J., Andralojc, P.J., Reynolds, M.P., Carmo-Silva, E., Parry, M.A.J. 03/2016 In: Journal of Experimental Botany. 67, 6, p. 1827-1838. 12 p.
Isoleucine 309 acts as a C4 catalytic switch that increases ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) carboxylation rate in flaveria
Whitney, S.M., Sharwood, R.E., Orr, D., White, S.J., Alonso, H., Galmés, J. 30/08/2011 In: Proceedings of the National Academy of Sciences. 108, 35, p. 14688-14693. 6 p.
Culture conditions affect the chemical composition of the exopolysaccharide synthesized by the fungus Aureobasidium pullulans
Orr, D., Zheng, W., Campbell, B.S., McDougall, B.M., Seviour, R.J. 08/2009 In: Journal of Applied Microbiology. 107, 2, p. 691-698. 8 p.
Realizing increased photosynthetic Efficiency for sustainable increases in Crop Yield
01/09/2015 → …
Society for Experimental Biology/Company of Biologists Travel Grant
Prize (including medals and awards)