Dr Douglas Orr

Senior Research Associate

Research Overview

My work as a member of the Photosynthesis Lab at LEC centres on the central enzyme of photosynthesis, Rubisco (Ribulose 1,5-bisphosphate carboxylase/oxygenase). As the gatekeeper of carbon entry into the biosphere, Rubisco serves the critical role of assimilating carbon from atmospheric CO2 for the plant to use in growth. However, Rubisco has undesirable characteristics which make it a major rate-limiter of photosynthesis, particularly in crop plants. As part of RIPE: Realizing Increased Photosynthetic Efficiency, we are characterising the biochemistry of this complex enzyme from a large number and broad range of species to better understand the variation present in nature.

Engineering photosynthesis: progress and perspectives
Orr, D.J., Pereira, A., da Fonseca Pereira, P., Pereira-Lima, I., Zsogon, A., Araujo, W. 26/10/2017
Review article

Extraction of Rubisco to Determine Catalytic Constants
Orr, D.J., Carmo-Silva, A.E. 8/08/2017 In: Photosynthesis: Methods and Protocols. Springer Science + Business Media
Chapter (peer-reviewed)

Uncertainty in measurements of the photorespiratory CO2 compensation point and its impact on models of leaf photosynthesis
Walker, B., Orr, D.J., Carmo-Silva, A.E., Parry, M.A.J., Bernacchi, C.J., Ort, D.R. 06/2017 In: Photosynthesis Research. 132, 3, p. 245-255. 11 p.
Journal article

Rubisco small subunits from the unicellular green alga Chlamydomonas complement Rubisco-deficient mutants of Arabidopsis
Atkinson, N., Leitão, N., Orr, D.J., Meyer, M., Carmo-Silva, A.E., Griffiths, H., Smith, A., McCormick, A. 04/2017 In: New Phytologist. 214, 2, p. 655-667. 13 p.
Journal article

Surveying Rubisco diversity and temperature response to improve crop photosynthetic efficiency
Orr, D.J., Melao Alcantara, A., Kapralov, M.V., Andralojc, P.J., Carmo-Silva, A.E., Parry, M.A.J. 3/10/2016 In: Plant Physiology. 172, 2, p. 707-717. 11 p.
Journal article

Rubisco catalytic properties of wild and domesticated relatives provide scope for improving wheat photosynthesis
Prins, A., Orr, D.J., Andralojc, P.J., Reynolds, M.P., Carmo-Silva, E., Parry, M.A.J. 03/2016 In: Journal of Experimental Botany. 67, 6, p. 1827-1838. 12 p.
Journal article

Isoleucine 309 acts as a C4 catalytic switch that increases ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) carboxylation rate in flaveria
Whitney, S.M., Sharwood, R.E., Orr, D., White, S.J., Alonso, H., Galmés, J. 30/08/2011 In: Proceedings of the National Academy of Sciences. 108, 35, p. 14688-14693. 6 p.
Journal article

Culture conditions affect the chemical composition of the exopolysaccharide synthesized by the fungus Aureobasidium pullulans
Orr, D., Zheng, W., Campbell, B.S., McDougall, B.M., Seviour, R.J. 08/2009 In: Journal of Applied Microbiology. 107, 2, p. 691-698. 8 p.
Journal article