Dr Nick Robinson


Research Overview

The Robinson laboratory studies fundamental cellular processes that are responsible for the maintenance of chromosomal integrity and protein homeostasis. The misregulation and mutation of these crucial pathways can result in a variety of pathological conditions including cancer, neurodegenerative and autoimmune diseases. The laboratory utilizes the experimental tractability of thermophilic archaeal species to investigate these essential processes. Although archaeal species are ancient microbes, components of the archaeal DNA repair and replication systems and protein degradation pathways display functional homology to their eukaryotic counterparts. Therefore, eukaryotic-like processes can be studied in these experimentally amenable organisms. Furthermore, the robust and stable archaeal protein homologues isolated from thermophilic species offer unparalleled benefits for the structural and biochemical study of these essential cellular pathways. By taking advantage of these streamlined archaeal systems, while employing a range of biochemical and structural approaches, the main aims of the laboratory are to further our understanding of the evolution and mechanistic action of the DNA replication-associated DNA repair events and also ubiquitin-like modifications and proteasomal degradation pathways.

Archaea, from obscurity to superhero microbes: 40 years of surprises and critical biological insights
Robinson, N.P. 14/12/2018 In: Emerging Topics in Life Sciences. 2, 4, 6 p.

Functional reconstruction of a eukaryotic-like E1/E2/(RING)E3 ubiquitylation cascade from an uncultured archaeon
James, R.H., Caceres, E.F., Escasinas, A., Alhasan, H., Howard, J.A., Deery, M.J., Ettema, T.J.G., Robinson, N.P. 24/10/2017 In: Nature Communications. 8, 15 p.
Journal article

Involvement of a eukaryotic-like ubiquitin-related modifier in the proteasome pathway of the archaeon Sulfolobus acidocaldarius
Anjum, R.S., Bray, S.M., Blackwood, J.K., Kilkenny, M.L., Coelho, M.A., Foster, B.M., Li, S., Howard, J.A., Pellegrini, L., Albers, S., Deery, M.J., Robinson, N.P. 8/09/2015 In: Nature Communications. 6, 15 p.
Journal article

Structure of the hexameric HerA ATPase reveals a mechanism of translocation-coupled DNA-end processing in archaea
Rzechorzek, N.J., Blackwood, J.K., Bray, S.M., Maman, J.D., Pellegrini, L., Robinson, N.P. 25/11/2014 In: Nature Communications. 5, p. 5506.
Journal article

Analysis of branched DNA replication and recombination intermediates from prokaryotic cells by two-dimensional (2D) native-native agarose gel electrophoresis
Robinson, N.P. 17/07/2013 In: Methods in Molecular Biology. 1054, p. 45-61. 17 p.
Journal article

End-resection at DNA double-strand breaks in the three domains of life
Blackwood, J.K., Rzechorzek, N.J., Bray, S.M., Maman, J.D., Pellegrini, L., Robinson, N.P. 1/02/2013 In: Biochemical Society Transactions. 41, 1, p. 314-320. 7 p.
Journal article

Structural and functional insights into DNA-end processing by the archaeal HerA helicase-NurA nuclease complex
Blackwood, J.K., Rzechorzek, N.J., Abrams, A.S., Maman, J.D., Pellegrini, L., Robinson, N.P. 04/2012 In: Nucleic Acids Research. 40, 7, p. 3183-96. 14 p.
Journal article

Extrachromosomal element capture and the evolution of multiple replication origins in archaeal chromosomes
Robinson, N.P., Bell, S.D. 3/04/2007 In: Proceedings of the National Academy of Sciences. 104, 14, p. 5806-5811. 6 p.
Journal article

Sister chromatid junctions in the hyperthermophilic archaeon Sulfolobus solfataricus
Robinson, N.P., Blood, K.A., McCallum, S.A., Edwards, P.A.W., Bell, S.D. 7/02/2007 In: EMBO Journal. 26, 3, p. 816-824. 9 p.
Journal article

Origins of DNA replication in the three domains of life
Robinson, N.P., Bell, S.D. 08/2005 In: FEBS Journal. 272, 15, p. 3757-3766. 10 p.
Journal article

Identification of two origins of replication in the single chromosome of the archaeon Sulfolobus solfataricus
Robinson, N.P., Dionne, I., Lundgren, M., Marsh, V.L., Bernander, R., Bell, S.D. 9/01/2004 In: Cell. 116, 1, p. 25-38. 14 p.
Journal article

DNA replication in the hyperthermophilic archaeon Sulfolobus solfataricus
Dionne, I., Robinson, N., McGeoch, A.T., Marsh, V.L., Reddish, A., Bell, S.D. 06/2003 In: Biochemical Society Transactions. 31, 3, p. 674-676. 3 p.
Journal article

Inactivation of Mre11 does not affect VSG gene duplication mediated by homologous recombination in Trypanosoma brucei
Robinson, N.P., McCulloch, R., Conway, C., Browitt, A., Barry, J.D. 19/07/2002 In: Journal of Biological Chemistry. 277, 29, p. 26185-26193. 9 p.
Journal article

Ku Is Important for Telomere Maintenance, but Not for Differential Expression of Telomeric VSG Genes, in African Trypanosomes.
Conway, C., McCulloch, R., Ginger, M.L., Robinson, N., Browitt, A., Barry, J.D. 14/06/2002 In: Journal of Biological Chemistry. 277, 24, p. 21269-21277. 9 p.
Journal article

Predominance of duplicative VSG gene conversion in antigenic variation in African trypanosomes
Robinson, N.P., Burman, N., Melville, S.E., Barry, J.D. 09/1999 In: Molecular and Cellular Biology. 19, 9, p. 5839-5846. 8 p.
Journal article