Dr Mick UrbaniakSenior Lecturer
My laboratory uses a combination of genetics, cell biology and proteomics approaches to understand biological systems at the molecular level. Our research is focused on tropical infectious diseases, primarily the clinically relevant parasite Trypanosoma brucei and related species such as Leishmania. We are studying how the parasites are able to sense and respond to their host environment, and aim to translate the results of this basic research into early-stage drug discovery.
Current projects in my laboratory include:-
- Probing dynamic phosphorylation in the post-transcriptional regulation of the Trypanosoma brucei cell cycle, funded by the BBSRC (BB/M009556/1)
- Investigating the origin of psychotic distrubances in stage II human African trypanosomiasis
- Iron sensing and uptake in the African trypanosome
- Stable isotope labelling for quantitative proteomics in the trypansomatids
Work in my laboratory is focused on the clinically relevant trypanosomatid parasites, primarily the African trypanosomes Trypanosoma brucei and Trypanosoma congolense, and the closely related American trypanosome Trypanosoma cruzi, and Leishmania species. These parasites are the causative agents of neglected tropical diseases that produce a substantial health and economic burden in endemic areas, and improved therapeutics are urgently needed. By examining the biology of these parasites we may uncover differences between the host and parasite biology that can be exploited to develop therapeutic treatments.
We are particularly interested in how African trypanosomes are able to sense and respond to its host environment, which is essential for their survival and virulence. The African trypanosome has a complex lifecycle requiring transmission by the insect vector the tsetse fly, propagation in a mammalian host, and reinfection of the tsetse fly. Trypanosomes are evolutionarily divergent eukaryotes and use exclusively post-transcriptional regulation of gene expression, making them an excellent model system to examine this process.
The laboratory uses a combination of genetics, cell biology and proteomics approaches to answer our research questions. We have pioneered stable isotopic labelling (SILAC) in T. brucei to enable global quantitative proteomic analysis, and established robust methods for quantifying changes in the phosphorylation state of the cell.
I am the Faculty Deputy Director for Natural Sciences, and teach on several modules within the Department of Biomedical and Life Sciences. I am a Fellow of the Higher Education Academy.
Currently I teach on the following modules:-
- BIOL272 Biochemical Techniques: Protein isolation and electrophoresis, combining theory with practical labs.
- BIOL301 Cell Signalling: Module Organiser, covering the Insulin signalling pathway.
- BIOL313 Protein Biochemistry: Protein structure determination; identifaction of proteins and post-translational modifications by mass spectrometry.
- BIOL314 Molecular & Biochemical Parasitology: Parasite glycobiology; drug discovery for Neglected Diseases.
I was appointed as a Lecturer within the Division of Biomedical and Life Sciences in September 2013, and promoted to Senior Lecture in 2018. My background is in Chemistry (BSc. and PhD.), but my subsequent postdoctoral research at the University of Dundee focussed on the biology of the trypanosomatids. I have a track record of successfully translating my basic research into early stage drug discovery with academic and industrial partners. I am a registered STEM ambassador, enabling me to engage in outreach activities to promote science in schools.
PhD Supervision Interests
My laboratory is seeking to recruit PhD and MRes students interested in the basic biology of Trypanosoma brucei and Leishmania species, which are the etiological agents of a number of diseases in developing countries. We can offer projects that use genetic, proteomic and biochemical approaches to study the parasites in vitro and in vivo. Please contact me for further details (No funded positions currently available).
Dynamic regulation of the Trypanosoma brucei transferrin receptor in response to iron starvation is mediated via the 3’UTR
Benz, C., Lo, W., Fathallah, N., Connor-Guscott, A., Benns, H.J., Urbaniak, M.D. 31/12/2018 In: PLoS ONE. 13, 12, 12 p.
Cyclin-dependent kinase 12, a novel drug target for visceral leishmaniasis
Wyllie, S., Thomas, M., Patterson, S., Crouch, S., De Rycker, M., Lowe, R., Gresham, S., Urbaniak, M.D., Otto, T.D., Stojanovski, L., Simeons, F.R.C., Manthri, S., MacLean, L.M., Zuccotto, F., Homeyer, N., Pflaumer, H., Boesche, M., Sastry, L., Connolly, P., Albrecht, S., Berriman, M., Drewes, G., Gray, D.W., Ghidelli-Disse, S., Dixon, S., Fiandor, J.M., Wyatt, P.G., Ferguson, M.A.J., Fairlamb, A.H., Miles, T.J., Read, K.D., Gilbert, I.H. 25/07/2018 In: Nature. 560, p. 192-197. 6 p.
Cell cycle synchronisation of Trypanosoma brucei by centrifugal counter-flow elutriation reveals the timing of nuclear and kinetoplast DNA replication
Benz, C., Dondelinger, F., McKean, P.G., Urbaniak, M.D. 14/12/2017 In: Scientific Reports. 7, 10 p.
Molecular control of irreversible bistability during trypanosome developmental commitment
Domingo Sananes, M.R., Szoor, B., Ferguson, M.A.J., Urbaniak, M., Matthews, K.R. 19/10/2015 In: Journal of Cell Biology. 211, 2, p. 455-468. 14 p.
The role of NSMCE2-dependent SUMO modification in the replication stress response
01/05/2019 → 30/04/2022
Hosting an academic visitor
Invited Seminar Speaker - Keele University
Invited speaker at UCLAN
Invited seminar speaker LSHTM
Invited Seminar at WCMP Glasgow
Invited Seminar at LSTHM
Invited speaker - ASTMH pre-meeting course
Member of the Royal Society of Biology
Election to learned society
Fellow of the Higher Education Academy
Election to learned society
Royal Society of Biology Ambassador
- DSI - Health