Dr Sarah AllinsonSenior Lecturer
Dr Allinson's work is aimed at gaining a better understanding of how cells respond to DNA damage. The two main areas of her research are (i) understanding how skin cells respond to damage caused by ultraviolet radiation and (ii) the mechanisms underlying the repair of breaks in the DNA backbone and factors that might affect their efficiency.
Cellular DNA is inherently unstable and is continually undergoing damage and degradation. Such damage can be caused by exposure to environmental carcinogens and also as a consequence of normal cellular metabolism, for example via the production of reactive oxygen species (ROS). My research is aimed at understanding the cellular response to DNA damage, with particular emphasis on the base excision repair (BER) and single strand break repair (SSBR) pathways.
Understanding how DNA repair is regulated at the level of single repair events and also within the cell as a whole can aid our understanding of inter-individual variation in cancer susceptibility and hopefully provide clues as to how repair in tumour cells may be attenuated to render cancer treatments more effective. With this aim in mind, there are a number of projects currently in progress in my lab.
Mechanistic studies of DNA repair
DNA repair pathways are multi-step processes involving a number of different enzymes and a network of protein-protein interactions. My research on DNA repair is aimed at understanding how the BER and SSBR pathways are co-ordinated and also how their efficiency might be affected by environmental carcinogens such as heavy metals. Through the detailed characterisation of repair of model DNA substrates by purified recombinant enzymes and cell extracts, facilitated by techniques such as site-directed mutagenesis, siRNA and protein-DNA interaction assays, we aim to gain a new mechanistic insight into how damaged DNA is repaired in vivo.
The cellular response to UVA exposure
We are currently investigating the effects of UVA exposure on human cells. Originally thought not to be a significant risk factor for skin cancer, UVA is now believed to contribute as much as 10-20% of the cancer-causing dose of sunlight. UVA induces DNA damage indirectly via photosensitiser-mediated production of ROS. One area of research that we are currently actively pursuing involves characterising the effects of UVA on stress response pathways and seeing how they differ from those induced by shorter wavelength UVB.
Work in my laboratory is currently funded by the BBSRC, Boots UK Limited and the Dowager Countess Eleanor Peel Trust.
BIOL437 Molecular Basis of Cancer: Module organiser and delivery of lectures/workshops etcBIOL463 Cell Biology Research Skills: Delivery of practical on flow cytometryBIOL353 Cancer: Module organiser and delivery of lectures/workshops/practicals etcBIOL253 Genetics: Delivery of lectures/workshops/practicals etc
BIOL272 Cell Biology Techniques: Delivery of two lecturesSpecial Study Module convenor on the Medicine MBChB degree
PhD Supervision Interests
I can offer PhD and MSc research projects on either molecular mechanisms of eukaryotic DNA repair or biological effects of ultraviolet radiation/skin cancer. Please contact me for further details (No funded positions currently available)
The role of innate immunity in the response to radiation-induced DNA damage in human skin cells
01/02/2019 → 30/04/2019
NWCR Equipment bid: Faxitron CellRad Irradiator
01/10/2018 → 31/03/2019
NWCR Summer Studentships 2018 and 2019
01/07/2018 → 30/09/2019
Proximity to Discover: Industry Engagement for Impact
31/03/2018 → 30/09/2019
01/12/2017 → 30/10/2020
North West Cancer Research 'We need to talk about skin cancer' event
Public Lecture/ Debate/Seminar