Dr Daniel Blackburn

Senior Research Associate

Research Overview

I´m very enthusiastic about the development of biotechnological approaches for using phytases to access phytate organic phosphorus pool present in soils and manures for plant nutrition. Since 2008 I have performed literature review and experimental work in the area of soil organic phosphorus abundance and availability, as affected by phytase enzymes and phytase producing microbes. I’m particularly interested content of phytase-labile organic P in manures and soils, as well as the environmental factors and enzyme properties affecting catalytic behavior of phytases in these environments.

Linking the depletion of rhizosphere phosphorus to the heterologous expression of a fungal phytase in Nicotiana tabacum as revealed by enzyme-labile P and solution 31P NMR spectroscopy
Giles, C.D., George, T.S., Brown, L.K., Mezeli, M., Shand, C.A., Richardson, A.E., Mackay, R., Wendler, R., Darch, T., Menezes-Blackburn, D., Cooper, P., Stutter, M.I., Lumsdon, D.G., Blackwell, M.S., Wearing, C., Zhang, H., Haygarth, P.M. 06/2017 In: Rhizosphere. 3, 1, p. 82-91. 10 p.
Journal article

A holistic approach to understanding the desorption of phosphorus in soils
Blackburn, D.M., Zhang, H., Stutter, M., Giles, C.D., Darch, T., George, T.S., Shand, C., Lumsdon, D., Blackwell, M.S.A., Wearing, C.L., Cooper, P., Wendler, R., Brown, L., Haygarth, P.M. 5/04/2016 In: Environmental Science and Technology. 50, 7, p. 3371-3381. 11 p.
Journal article

Phytase-producing Bacillus sp. inoculation increases phosphorus availability in cattle manure
Blackburn, D.M., Inostroza, N.G., Gianfreda, L., Greiner, R., Mora, M.L., Jorquera, M.A. 03/2016 In: Journal of Soil Science and Plant Nutrition . 16, 1, p. 200-210. 11 p.
Journal article

Performance of Seven Commercial Phytases in an in Vitro Simulation of Poultry Digestive Tract
Menezes-Blackburn, D., Gabler, S., Greiner, R. 15/07/2015 In: Journal of Agricultural and Food Chemistry. 63, 27, p. 6142-6149. 8 p.
Journal article

Enzymes used in animal feed: leading technologies and forthcoming developments
Blackburn, D.M., Greiner, R. 05/2015 In: Functional polymers in food science. Calabria : Wiley p. 47-73. 24 p. ISBN: 9781118595183.

A novel phosphorus biofertilization strategy using cattle manure treated with phytase-nanoclay complexes
Menezes-Blackburn, D., Jorquera, M.A., Gianfreda, L., Greiner, R., de la Luz Mora, M. 05/2014 In: Biology and Fertility of Soils. 50, 4, p. 583-592. 10 p.
Journal article

Production of partially phosphorylated myo-inositol phosphates using phytases immobilised on magnetic nanoparticles
Greiner, R., Konietzny, U., Blackburn, D.M., Jorquera, M.A. 08/2013 In: Bioresource Technology. 142, p. 375-383. 9 p.
Journal article

Phytases and phytase-labile organic phosphorus in manures and soils
Menezes-Blackburn, D., Jorquera, M.A., Greiner, R., Gianfreda, L., de la Luz Mora, M. 2013
Literature review

Activity stabilization of Aspergillus niger and Escherichia coli phytases immobilized on allophanic synthetic compounds and montmorillonite nanoclays
Menezes-Blackburn, D., Jorquera, M., Gianfreda, L., Rao, M., Greiner, R., Garrido, E., de la Luz Mora, M. 10/2011 In: Bioresource Technology. 102, 20, p. 9360-7. 8 p.
Journal article

Phosphorus and Nitrogen Fertilization Effect on Phosphorus Uptake and Phosphatase Activity in Ryegrass and Tall Fescue Grown in a Chilean Andisol
Paredes, C., Menezes-Blackburn, D., Cartes, P., Gianfreda, L., Luz Mora, M. 05/2011 In: Eurasian Soil Science. 176, 5, p. 245-251. 7 p.
Journal article

Identification of β-propeller phytase-encoding genes in culturable Paenibacillus and Bacillus spp. from the rhizosphere of pasture plants on volcanic soils
Jorquera, M.A., Crowley, D.E., Marschner, P., Greiner, R., Fernández, M.T., Romero, D., Menezes-Blackburn, D., De La Luz Mora, M. 1/01/2011 In: FEMS Microbiology Ecology. 75, 1, p. 163-172. 10 p.
Journal article

Indole acetic acid and phytase activity produced by rhizosphere bacilli as affected by pH and metals
Acuna, J.J., Jorquera, M.A., Martinez, O.A., Menezes-Blackburn, D., Fernandez, M.T., Marschner, P., Greiner, R., Mora, M.L. 2011 In: Journal of Soil Science and Plant Nutrition . 11, 3, p. 1-12. 12 p.
Journal article