Dr Daniel BlackburnSenior Research Associate
I´m very enthusiastic about the development of biotechnological approaches for using phytases to access phytate organic phosphorus pool present in soils and manures for plant nutrition. Since 2008 I have performed literature review and experimental work in the area of soil organic phosphorus abundance and availability, as affected by phytase enzymes and phytase producing microbes. I’m particularly interested content of phytase-labile organic P in manures and soils, as well as the environmental factors and enzyme properties affecting catalytic behavior of phytases in these environments.
My past work has been developed in cooperation Universidad de La Frontera (UFRO-Chile), University of Naples (UNINA, Italy) and Max Rubner-Institut (MRI, Germany). To date, I have leaded and coauthored 8 papers published in high impact factor ISI indexed journals and one book chapter. Please visit my ResearchGate profile to see my complete list of publications.
I’m currently working as PDRA in the OPUS project, recently funded by the Biotechnology and Biological Sciences Research Council (BBSRC) to explore cropping strategies to target the use of recalcitrant soil Po. This consortium includes researchers from Lancaster University (Lancaster, UK), James Hutton Institute (Aberdeen and Dundee, UK) and Rothamsted Research (Devon, UK). In this project we will investigate the use of low molecular weight organic acid anions (LMWOAA) and phytases to access soil organic phosphorus. Experimental techniques employed will include plant phytase exudation analysis, organic phosphorus analysis using diffusive gradients in thin films (DGT), HPLC, enzyme hydrolysis and 31P nuclear magnetic resonance.
A holistic approach to understanding the desorption of phosphorus in soils
Blackburn, D.M., Zhang, H., Stutter, M., Giles, C.D., Darch, T., George, T.S., Shand, C., Lumsdon, D., Blackwell, M.S.A., Wearing, C.L., Cooper, P., Wendler, R., Brown, L., Haygarth, P.M. 5/04/2016 In: Environmental Science and Technology. 50, 7, p. 3371-3381. 11 p.
Phytase-producing Bacillus sp. inoculation increases phosphorus availability in cattle manure
Blackburn, D.M., Inostroza, N.G., Gianfreda, L., Greiner, R., Mora, M.L., Jorquera, M.A. 03/2016 In: Journal of Soil Science and Plant Nutrition . 16, 1, p. 200-210. 11 p.
Production of partially phosphorylated myo-inositol phosphates using phytases immobilised on magnetic nanoparticles
Greiner, R., Konietzny, U., Blackburn, D.M., Jorquera, M.A. 08/2013 In: Bioresource Technology. 142, p. 375-383. 9 p.
Activity stabilization of Aspergillus niger and Escherichia coli phytases immobilized on allophanic synthetic compounds and montmorillonite nanoclays
Menezes-Blackburn, D., Jorquera, M., Gianfreda, L., Rao, M., Greiner, R., Garrido, E., de la Luz Mora, M. 10/2011 In: Bioresource Technology. 102, 20, p. 9360-7. 8 p.
Identification of β-propeller phytase-encoding genes in culturable Paenibacillus and Bacillus spp. from the rhizosphere of pasture plants on volcanic soils
Jorquera, M.A., Crowley, D.E., Marschner, P., Greiner, R., Fernández, M.T., Romero, D., Menezes-Blackburn, D., De La Luz Mora, M. 1/01/2011 In: FEMS Microbiology Ecology. 75, 1, p. 163-172. 10 p.