Location: Faraday A71 Mass Spectrometry
The Shimadzu LCMS-IT-TOF mass spectrometer is fronted by a Shimadzu NexeraX2 UHPLC system; the instrument is designed for high-resolution accurate mass MSn experiments.
Coupled to HPLC/UHPLC separation the LCMS-IT-TOF combines a QIT (ion trap) and TOF (time-of-flight) mass spectrometer system. The unique combination of ion-trap and time-of-flight technologies allows mass spec fragmentation experiments, MSn, up to MS10 with a high-accuracy mass assignment of precursor and product ions.
Location: cTAP B06 Instrument Room
The Shimadzu LCMS-8040 triple quadrupole mass spectrometer fronted by a Shimadzu NexeraX2 UHPLC system; for SRM/MRM quantitative LCMS analysis.
The LCMS-8040 enables high sensitivity mass analysis of HPLC/UHPLC separations. A combined MS probe enables simultaneous ESI and APCI for highly sensitive and specific quantitative analysis of samples. Triple quad mass analysis is common in applications such as omics studies, environmental and biological analysis requiring very low levels of detection and quantitation.
Shimadzu NexeraX2 UHPLC-PDA
Location: one in Faraday A70 Mass Spectrometry and one in cTAP B01 Laboratory
Ultra-high performance liquid chromatography with PDA detection, and optional AccuSpot MALDI spotting robot.
For the separation, identification and quantitation of components dissolved in liquid samples, the Shimadzu NexeraX2 system is capable of ultra-high pressure chromatography of a range of sample types. High sensitivity, multi-wavelength analysis is achieved with the PDA detector. Analysis is routinely conducted using reverse-phase conditions and consumables, however, with appropriate selection of mobile and stationary phases alternative LC experiments can be conducted.
Location: Faraday A70 Mass Spectrometry
Gel permeation/size exclusion chromatography specific equipment with detection via RID, Wyatt Technologies MALS and viscometry detectors. Also available is an AccuSpot MALDI spotting robot.
Gel permeation chromatography (GPC), or size exclusion chromatography (SEC), equipment for the measurement of molecular weight values of synthetic polymers and biomolecules (such as proteins, etc.). The combination of size fractionation with refractive index (RID), light scattering (MALS) and viscometry detection allows the on-line measurement of molecular weight, molecular weight distribution and molecular interactions without the need for extensive instrument/column calibration.
Basic analysis routinely conducted by this instrument for polymer analysis uses THF solvent, a broad molecular weight column, and the three detector system for the analysis of molecular weight and molecular weight distribution. Typical sample submission requires 1–10 mg of sample dissolved in 1 ml mobile phase.
Agilent 6120B LCMS
The Agilent 1260 Infinity HPLC with 6120B Single Quad MS offers a robust, versatile, sensitive and selective analysis of a broad range of compounds for routine characterization of complex mixtures. A quaternary gradient HPLC coupled with a choice of chromatographic stationary phases (C8, C18, phenyl-hexyl, SB-aq) and the specificity of MS detection enables LCMS to cover a broad range of analytical requirements for liquid soluble, polar or non-polar compounds.
Agilent 1220 Infinity HPLC
Location: Faraday B32 Teaching Laboratory
The Agilent 1220 Infinity HPLC instrument comprises a binary gradient solvent pump and single-wavelength UV-vis detection. Robust, reproducible HPLC analysis is provided with a single unit capable of full automation.
HPLC analysis is conducted using a range of available column chemistries including C8, C18, phenyl-hexyl, SB-aq phases. Samples submitted in a range of concentrations from <1 mg/ml dissolved in an analytical solvent. Instrument detection limited to UV-vis spectroscopy, but this is likely to cover most analytical requirements.
Thermo Scientific Dionex ICS-5000+ HPIC
Location: cTAP B01 Laboratory
Thermo Scientific Dionex ICS-5000+ Reagent Free Ion Chromatography dual microbore analysis for anions (organic, inorganic, transition metal oxides) and cations.
The ICS-5000+, with all-PEEK flow path capable of operating at high pressures (2000-3000 psi), for the analysis of anions and cations in sample mixtures. Reagent-free eluent generation, ion suppression and conductivity detection offer high sensitivity analysis for a range of ions. Dual IC channels, complete with separate pumps, eluent generation, stationary phase and detection enables simultaneous cation and anion detection of the same sample.
The modular system with all-PEEK flow path contains a dual system to run two different analyses concurrently on a single sample. The system is configured for analysis with dual pump, Reagent-free eluent generation, microbore (3–7 mm i.d.) separation, eluent ion suppression and conductivity detection. Aqueous components detected routinely in the parts per million range. Organic mobile phase analysis is also possible with instrument re-configuration.