Dr Lorna Ashton

Lecturer

Research Overview

My area of interest is the advancement of Raman spectroscopy as an analytical tool for monitoring physiochemical properties of molecules. My research involves combining Raman spectroscopy and chemometric methods for the identification of structural changes due to post-translational modifications, protein-protein interactions, ligand binding and changes in protein stability as well as working towards on-line monitoring of biopharmaceutical products, substrates and by-products. Furthermore, I am developing Raman micro-spectroscopy techniques to image live cells during abiotic perturbations.

Deep convolutional neural networks for Raman spectrum recognition: a unified solution
Liu, J., Osadchy, M., Ashton, L., Foster, M., Solomon, C.J., Gibson, S.J. 28/09/2017 In: The Analyst. 8 p.
Journal article

Detection of glycosylation and iron-binding protein modifications using Raman spectroscopy
Ashton, L., Brewster, V., Correa, E., Goodacre, R. 7/03/2017 In: The Analyst. 142, 5, p. 808-814. 7 p.
Journal article

Two-dimensional codistribution spectroscopy applied to UVRR and ROA investigations of biomolecular transitions
Ramer, G., Ashton, L. 15/11/2016 In: Journal of Molecular Structure. 1124, p. 173-179. 7 p.
Journal article

Raman spectroscopy: an evolving technique for live cell studies
Smith, R., Wright, K.L., Ashton, L. 21/06/2016
Literature review

Using Raman spectroscopy to characterize biological materials
Butler, H.J., Ashton, L., Bird, B., Cinque, G., Curtis, K., Dorney, J., Esmonde-White, K., Fullwood, N.J., Gardner, B., Martin-Hirsch, P.L., Walsh, M.J., McAinsh, M.R., Stone, N., Martin, F.L. 04/2016 In: Nature Protocols. 11, 4, p. 664-687. 24 p.
Journal article

Detection of protein glycosylation using tip enhanced Raman scattering
Cowcher, D., Deckert-Gaudig, T., Brewster, V., Ashton, L., Deckert, V., Goodacre, R. 16/02/2016 In: Analytical Chemistry. 88, 4, p. 2105-2112. 8 p.
Journal article

Detection of early stage changes associated with adipogenesis using Raman spectroscopy under aseptic conditions
Mitchell, A., Ashton, L., Yang, X.B., Goodacre, R., Smith, A., Kirkham, J. 6/10/2015 In: Cytometry Part A.
Journal article

Aseptic Raman spectroscopy can detect changes associated with the culture of human dental pulp stromal cells in osteoinductive culture
Mitchell, A., Ashton, L., Yang, X.B., Goodacre, R., Tomlinson, M.J., Smith, A., Kirkham, J. 10/09/2015 In: The Analyst. 140, 21, p. 7347-7354. 8 p.
Journal article

Making colourful sense of Raman images of single cells
Ashton, L., Hollyowood, K., Goodacre, R. 21/03/2015 In: The Analyst. 140, 6, p. 1852-1858. 7 p.
Journal article

UV resonance Raman spectroscopy: a process analytical tool for host cell DNA and RNA dynamics in mammalian cell lines
Ashton, L., Hogwood, C.E.M., Tait, A.S., Kuligowski, J., Smales, C.M., Bracewell, D.G., Dickson, A.J., Goodacre, R. 02/2015 In: Journal of Chemical Technology and Biotechnology. 90, 2, p. 237-243. 7 p.
Journal article

Monitoring antibody aggregation in early drug development using Raman spectroscopy and perturbation-correlation moving windows
Gómez de la Cuesta, R., Goodacre, R., Ashton, L. 18/11/2014 In: Analytical Chemistry. 86, 22, p. 11133-11140. 8 p.
Journal article

Investigation of DMSO-induced conformational transitions in human serum albumin using two-dimensional Raman optical activity spectroscopy
Batista, A.N.L., Batista, J.M., Ashton, L., Bolzani, V.S., Furlan, M., Blanch, E.W. 09/2014 In: Chirality. 26, 9, p. 497-501. 5 p.
Journal article

Raman spectroscopy can characterise the early stages of osteoinduction in dental pulp stromal cells
Mighell, A., Ashton, L., Yang, X., Smith, A., Kirkham, J. 06/2014
Meeting abstract

Understanding glycoprotein behaviours using Raman and Raman optical activity spectroscopies: characterising the entanglement induced conformational changes in oligosaccharide chains of mucin
Ashton, L., Pudney, P.D.A., Blanch, E.W., Yakubov, G.E. 11/2013 In: Advances in Colloid and Interface Science. 199-200, p. 66-77. 12 p.
Journal article

The challenge of applying Raman spectroscopy to monitor recombinant antibody production
Ashton, L., Xu, Y., Brewster, V.L., Cowcher, D.P., Sellick, C.A., Dickson, A.J., Stephens, G.M., Goodacre, R. 26/09/2013 In: The Analyst. 138, 22, p. 6977-6985. 9 p.
Journal article

Monitoring Guanidinium-Induced Structural Changes in Ribonuclease Proteins Using Raman Spectroscopy and 2D Correlation Analysis
Brewster, V.L., Ashton, L., Goodacre, R. 2/04/2013 In: Analytical Chemistry. 85, 7, p. 3570-3575. 6 p.
Journal article

Illuminating disease and enlightening biomedicine: Raman spectroscopy as a diagnostic tool
Ellis, D.I., Cowcher, D.P., Ashton, L., O'Hagan, S., Goodacre, R. 2013
Literature review

The importance of protonation in the investigation of protein phosphorylation using Raman spectroscopy and Raman optical activity
Ashton, L., Johannessen, C., Goodacre, R. 15/10/2011 In: Analytical Chemistry. 83, 20, p. 7978-7983. 6 p.
Journal article

Raman spectroscopy: lighting up the future of microbial identification
Ashton, L., Lau, K., Winder, C.L., Goodacre, R. 09/2011 In: Future Microbiology. 6, 9, p. 991-997. 7 p.
Journal article

Monitoring the glycosylation status of proteins using Raman spectroscopy
Brewster, V.L., Ashton, L., Goodacre, R. 1/08/2011 In: Analytical Chemistry. 83, 15, p. 6074-6081. 8 p.
Journal article

Structural, spectroscopic and redox properties of uranyl complexes with a maleonitrile containing ligand
Hardwick, H.C., Royal, D.S., Helliwell, M., Pope, S.J.A., Ashton, L., Goodacre, R., Sharrad, C.A. 2011 In: Dalton Transactions. 40, 22, p. 5939-5952. 14 p.
Journal article

pH-induced conformational transitions in alpha-lactalbumin investigated with two-dimensional Raman correlation variance plots and moving windows
Ashton, L., Blanch, E.W. 16/06/2010 In: Journal of Molecular Structure. 974, 1-3, p. 132-138. 7 p.
Journal article

Susceptibility of different proteins to flow-induced conformational changes monitored with Raman spectroscopy
Ashton, L., Dusting, J., Imomoh, E., Balabani, S., Blanch, E.W. 17/02/2010 In: Biophysical Journal. 98, 4, p. 707-714. 8 p.
Journal article

Shear-induced unfolding of lysozyme monitored in situ
Ashton, L., Dusting, J., Imomoh, E., Balabani, S., Blanch, E.W. 20/05/2009 In: Biophysical Journal. 96, 10, p. 4231-4236. 6 p.
Journal article

Investigations of conformational transitions in proteins and RNA using 2DCOS Raman and 2DCOS Raman optical activity spectroscopies
Ashton, L., Hobro, A., Conn, G.L., Rouhi, M., Blanch, E.W. 30/07/2008 In: Journal of Molecular Structure. 883-884, p. 187-194. 8 p.
Journal article

Investigation of polypeptide conformational transitions with two-dimensional Raman optical activity correlation analysis, applying autocorrelation and moving window approaches
Ashton, L., Blanch, E.W. 05/2008 In: Applied Spectroscopy. 62, 5, p. 469-475. 7 p.
Journal article

Two-dimensional Raman and Raman optical activity correlation analysis of the alpha-helix-to-disordered transition in poly(L-glutamic acid)
Ashton, L., Barron, L.D., Hecht, L., Hyde, J., Blanch, E.W. 05/2007 In: The Analyst. 132, 5, p. 468-479. 12 p.
Journal article

Application of two-dimensional correlation analysis to Raman optical activity
Ashton, L., Czarnik-Matusewicz, B., Blanch, E.W. 6/11/2006 In: Journal of Molecular Structure. 799, 1-3, p. 61-71. 11 p.
Journal article

Potential pitfalls concerning visualization of the 2D results
Czarnik-Matusewicz, B., Pilorz, S., Ashton, L., Blanch, E.W. 6/11/2006 In: Journal of Molecular Structure. 799, 1-3, p. 253-258. 6 p.
Journal article

Two-dimensional correlation analysis of Raman optical activity data on the α-helix-to-β-sheet transition in poly(L-lysine)
Ashton, L., Barron, L.D., Czarnik-matusewicz, B., Hecht, L., Hyde, J., Blanch, E.W. 10/05/2006 In: Molecular Physics. 104, 9, p. 1429-1445. 17 p.
Journal article

Monitoring the mode of action of antibiotics using Raman spectroscopy: investigating subinhibitory effects of amikacin on Pseudomonas aeruginosa
López-Diez, E.C., Winder, C.L., Ashton, L., Currie, F., Goodacre, R. 1/05/2005 In: Analytical Chemistry. 77, 9, p. 2901-2906. 6 p.
Journal article